Learning Objectives

Introduction to RStudio

Throughout this lesson, we’re going to teach you some of the fundamentals of the R language as well as some best practices for organising code for scientific projects that will make your life easier.

We’ll be using RStudio: a free, open source R integrated development environment. It provides a built in editor, works on all platforms (including on servers) and provides many advantages such as integration with version control and project management.

Basic layout

When you first open RStudio, you will be greeted by three panels:

Once you open files, such as R scripts, an editor panel will also open in the top left.

Work flow within RStudio

There are two main ways one can work within RStudio.

  1. Test and play within the interactive R console
  1. Start writing in an .R file and use RStudio’s command / short cut to push current line, selected lines or modified lines to the interactive R console.

Tip: Running segments of your code

RStudio offers you great flexibility in running code from within the editor window. There are buttons, menu choices, and keyboard shortcuts. To run the current line, you can 1. click on the Run button just above the editor panel, or 2. select “Run Lines” from the “Code” menu, or 3. hit Ctrl-Enter in Windows or Linux or Command-Enter on OS X. (This shortcut can also be seen by hovering the mouse over the button). To run a block of code, select it and then Run. If you have modified a line of code within a block of code you have just run, there is no need to reselct the section and Run, you can use the next button along, Re-run the previous region. This will run the previous code block inculding the modifications you have made.

Introduction to R

Much of your time in R will be spent in the R interactive console. This is where you will run all of your code, and can be a useful environment to try out ideas before adding them to an R script file. This console in RStudio is the same as the one when you open up the basic R GUI.

The first thing you will see in the R interactive session is a bunch of information, followed by a “>” and a blinking cursor. It operates on the idea of a “Read, Evaluate, Print loop” (REPL): you type in commands, R tries to execute them, and then returns a result.

Using R as a calculator

The simplest thing you could do with R is do arithmetic:

1 + 100
## [1] 101

And R will print out the answer, with a preceding “[1]”. Don’t worry about this for now, we’ll explain that later. For now think of it as indicating ouput.

If you type in an incomplete command, R will wait for you to complete it:

> 1 +
+

Any time you hit return and the R session shows a “+” instead of a “>”, it means it’s waiting for you to complete the command. If you want to cancel a command you can simply hit “Esc” and RStudio will give you back the “>” prompt.

Tip: Cancelling commands

Cancelling a command isn’t just useful for killing incomplete commands: you can also use it to tell R to stop running code (for example if its taking much longer than you expect), or to get rid of the code you’re currently writing.

When using R as a calculator, the order of operations is the same as you would have learnt back in school.

From highest to lowest precedence:

3 + 5 * 2
## [1] 13

Use parentheses to group operations in order to force the order of evaluation if it differs from the default, or to make clear what you intend.

(3 + 5) * 2
## [1] 16

This can get unwieldy when not needed, but clarifies your intentions. Remember that others may later read your code.

(3 + (5 * (2 ^ 2))) # hard to read
3 + 5 * 2 ^ 2       # clear, if you remember the rules
3 + 5 * (2 ^ 2)     # if you forget some rules, this might help

The text after each line of code is called a “comment”. Anything that follows after the hash (or octothorpe) symbol # is ignored by R when it executes code.

Really small or large numbers get a scientific notation:

2 / 10000
## [1] 2e-04

Which is shorthand for “multiplied by 10^XX”. So 2e-4 is shorthand for 2 * 10^(-4).

You can write numbers in scientific notation too:

5e3  # Note the lack of minus here
## [1] 5000

Functions

Most of R’s functionality comes from its functions. A function takes zero, one or multiple arguments, depending on the function, and returns a value. To call a function enter it’s name followed by a pair of brackets - include any arguments in the brackets.

log(10)
## [1] 2.302585

To find out more about a function called function_name type ?function_name. To search for the functions associated with a topic type ??topic or ??"multiple topics". As well as providing a detailed description of the command and how it works, scrolling ot the bottom of the help page will usually show a collection of code examples which illustrate command usage.

Exercise 1 Which function calculates sums? And what arguments does it take?

Arguments

The documentation for log indicates that the function requires an argument x that is a vector of numeric (real) or complex numbers and an argument base which is the base of the logarithm.

Exercise 2 What kind of logarithm does the log function take by default?

When calling a function its arguments can be specified using positional and/or named matching.

log(x = 10, base = 2)
## [1] 3.321928
log(10, 2)
## [1] 3.321928
log(2, 10)
## [1] 0.30103

Mathematical functions

R has many built in mathematical functions.

sin(1)  # trigonometry functions
## [1] 0.841471
log(1)  # natural logarithm
## [1] 0
log10(10) # base-10 logarithm
## [1] 1
exp(0.5) # e^(1/2)
## [1] 1.648721

Don’t worry about trying to remember every function in R. You can simply look them up on google, or if you can remember the start of the function’s name, use the tab completion in RStudio.

This is one advantage that RStudio has over R on its own, it has autocompletion abilities that allow you to more easily look up functions, their arguments, and the values that they take.

Comparing things

We can also do comparison in R:

1 == 1  # equality (note two equals signs, read as "is equal to")
## [1] TRUE
1 != 2  # inequality (read as "is not equal to")
## [1] TRUE
1 <  2  # less than
## [1] TRUE
1 <= 1  # less than or equal to
## [1] TRUE
1 > 0  # greater than
## [1] TRUE
1 >= -9 # greater than or equal to
## [1] TRUE

Tip: Comparing Numbers

A word of warning about comparing numbers: you should never use == to compare two numbers unless they are integers (a data type which can specifically represent only whole numbers).

Computers may only represent decimal numbers with a certain degree of precision, so two numbers which look the same when printed out by R, may actually have different underlying representations and therefore be different by a small margin of error (called Machine numeric tolerance).

Instead you should use the all.equal function.

Further reading: http://floating-point-gui.de/

Variables and assignment

We can store values in variables by giving them a name, and using the assignment operator <- (To save finger strokes, type Alt-):

x <- 1 / 40

Notice that assignment does not print a value. Instead, we stored it for later in something called a variable. x now contains the value 0.025:

x
## [1] 0.025

Look for the Environment tab in one of the panes of RStudio, and you will see that x and its value have appeared. Our variable x can be used in place of a number in any calculation that expects a number:

log(x)
## [1] -3.688879

Notice also that variables can be reassigned:

x <- 100

x used to contain the value 0.025 and and now it has the value 100.

Assignment values can contain the variable being assigned to:

x <- x + 1 #notice how RStudio updates its description of x on the top right tab

The right hand side of the assignment can be any valid R expression. The right hand side is fully evaluated before the assignment occurs.

Exercise 3 Create an object called x with the value 7. What is the value of x^x. Save the value in a object called i. If you assign the value 20 to the object x does the value of i change? What does this indicate about how R assigns values to objects?

Variable names can contain letters, numbers, underscores and periods. They cannot start with a number nor contain spaces at all. Different people use different conventions for long variable names, these include

What you use is up to you, but be consistent.

It is also possible to use the = operator for assignment:

x = 1 / 40

But this is much less common among R users. The most important thing is to be consistent with the operator you use. There are occasionally places where it is less confusing to use <- than =, and it is the most common symbol used in the community. So the recommendation is to use <-.

Managing your environment

There are a few useful commands you can use to interact with the R session.

ls will list all of the variables and functions stored in the global environment (your working R session):

ls()
[1] "x"   "y"

Note here that we didn’t given any arguments to ls, but we still needed to give the parentheses to tell R to call the function.

If we type ls by itself, R will print out the source code for that function!

You can use rm to delete objects you no longer need:

rm(x)

If you have lots of things in your environment and want to delete all of them, you can pass the results of ls to the rm function:

rm(list = ls())

In this case we’ve combined the two. Just like the order of operations, anything inside the innermost parentheses is evaluated first, and so on.

In this case we’ve specified that the results of ls should be used for the list argument in rm.

Tip: Warnings vs. Errors

Pay attention when R does something unexpected! Errors, like above, are thrown when R cannot proceed with a calculation. Warnings on the other hand usually mean that the function has run, but it probably hasn’t worked as expected.

In both cases, the message that R prints out usually give you clues how to fix a problem.

Challenge 1

Which of the following are valid R variable names?

min_height
max.height
_age
.mass
MaxLength
min-length
2widths
celsius2kelvin

Challenge 2

What will be the value of each variable after each statement in the following program?

mass <- 47.5
age <- 122
mass <- mass * 2.3
age <- age - 20

Challenge 3

Run the code from the previous challenge, and write a command to compare mass to age. Is mass larger than age?

Challenge 4

Clean up your working environment by deleting the mass and age variables.

Project management with RStudio

Introduction

The scientific process is naturally incremental, and many projects start life as random notes, some data, some code, then a report or manuscript, and eventually everything is a bit mixed together.

It’s pretty easy to get data scattered among many different folders, with multiple versions.

There are many reasons why we should avoid this:

  1. It is really hard to tell which version of your data is the original and which is the modified;
  2. It gets really messy because it mixes files with various extensions together;
  3. It probably takes you a lot of time to actually find things, and relate the correct figures to the exact files/code that has been used to generate it;

A good project layout will ultimately make your life easier:

  • It will help ensure the integrity of your data;
  • It makes it simpler to share your code with someone else (a lab-mate, collaborator, or supervisor);
  • It allows you to easily upload your code with your manuscript submission;
  • It makes it easier to pick the project back up after a break.

A possible solution

Fortunately, there are tools and packages which can help you manage your work effectively.

One of the most powerful and useful aspects of RStudio is its project management functionality. We’ll be using this today to create a self-contained, reproducible project.

Challenge 5: Creating a self-contained project

We’re going to create a new project in RStudio:

  1. Click the “File” menu button, then “New Project”.
  2. Click “New Directory”.
  3. Click “Empty Project”.
  4. Type in the name of the directory to store your project, e.g. “r_course”.
  5. Click the “Create Project” button.

Now when we start R in this project directory, or open this project with RStudio, all of our work on this project will be entirely self-contained in this directory.

Best practices for project organisation

Although there is no “best” way to lay out a project, there are some general principles to adhere to that will make project management easier:

Treat data as read only

This is probably the most important goal of setting up a project. Data is typically time consuming and/or expensive to collect. Working with them interactively (e.g., in Excel) where they can be modified means you are never sure of where the data came from, or how it has been modified since collection. It is therefore a good idea to treat your data as “read-only”.

Data Cleaning

In many cases your data will be “dirty”: it will need significant preprocessing to get into a format R (or any other programming language) will find useful. This task is sometimes called “data munging”. I find it useful to store these scripts in a separate folder, and create a second “read-only” data folder to hold the “cleaned” data sets.

Treat generated output as disposable

Anything generated by your scripts should be treated as disposable: it should all be able to be regenerated from your scripts.

There are lots of different ways to manage this output. I find it useful to have an output folder with different sub-directories for each separate analysis. This makes it easier later, as many of my analyses are exploratory and don’t end up being used in the final project, and some of the analyses get shared between projects.

Separate function definition and application

The most effective way I find to work in R, is to play around in the interactive session, then copy commands across to a script file when I’m sure they work and do what I want. You can also save all the commands you’ve entered using the history command, but I don’t find it useful because when I’m typing its 90% trial and error.

When your project is new and shiny, the script file usually contains many lines of directly executed code. As it matures, reusable chunks get pulled into their own functions. It’s a good idea to separate these into separate folders; one to store useful functions that you’ll reuse across analyses and projects, and one to store the analysis scripts.

Save the data in the data directory

Now we have a good directory structure we will now place/save the data file in the data/ directory.

Challenge 6

Download the gapminder data from here.

  1. Download the file (CTRL + S, right mouse click -> “Save as”, or File -> “Save page as”)
  2. Make sure it’s saved under the name gapminder-FiveYearData.csv
  3. Save the file in the data/ folder within your project.

We will load and inspect these data later.